Document Details
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Document Type |
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Thesis |
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Document Title |
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Extraction of Phytochemicals and their Use in the Preparation of Metallic Nanoparticles and Metal Oxides for Enhanced Catalytic and Antimicrobial Applications استخلاص المواد الكيميائية النباتية واستخدامها في تحضير الجسيمات النانوية المعدنية وأكاسيد المعادن لتعزيز استخدام المواد الحفازة ومضادات الميكروبات |
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Subject |
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Faculty of Science |
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Document Language |
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Arabic |
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Abstract |
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In terms of reduced toxicity, the biologically inspired green synthesis of nanoparticles has emerged as a promising alternative to chemically and physically fabricated nanoparticles. In addition, the green synthesis of nanoparticles is simple, environmental friendliness, safety, and cost-effectiveness. In the present work, the biocompatible and environmentally friendly aqueous extract of black dried lemon peel and Cynara cardunculus were used as a reducing and capping agent for green synthesis of zinc oxide nanoparticles (LP-ZnO NPs) and silver nanoparticles (CC-Ag NPs) respectively. The surface morphology, elemental composition, crystalline properties, size, optical properties, the role of functional groups in stabilization, capping, and the thermal stability of the new nanoparticles were investigated using X-ray diffraction spectroscopy (XRD), Fourier transform infrared spectroscopy (FTIR), UV-visible spectroscopy, scanning electron microscopy energy-dispersive X-ray spectroscopy (SEM-EDX), transmission electron microscopy (TEM), and thermogravimetric (TGA). Photodegradation of antibiotic ciprofloxacin was observed in the presence of UV light via LP-ZnO NPs (serving as photocatalyst). In addition, in optimal reaction media, the biogenic LP-ZnO NPs retained improved photocatalytic performance toward ciprofloxacin (CIP). Meanwhile, in the photodegradation process of CIP molecules via ZnO as a photocatalyst, the optimum catalytic dose and concentration of CIP molecules were attained at 10 mg, 2 x10-5 M, and pH 8, respectively. The LP-ZnO NPs exhibited strong photocatalytic activity toward ciprofloxacin and retained more than 80% CIP degradation within 160 min. On the other side, the antifungal activity of CC-AgNPs against C. auris MRL6057 was study. The minimum inhibitory concentrations (MIC) and minimum fungicidal concentrations (MFC) were 50.0 µg/mL and 100.0 µg/mL respectively. The cell count and viability assay confirmed the fungicidal potential of CC-AgNPs. Further, the analysis showed that CC-AgNPs could induce apoptosis and G2/M phase cell cycle arrest in C. auris MRL6057. The CC-AgNPs were responsible for the induction of mitochondrial toxicity. TUNEL assay results revealed that higher concentrations of CC-AgNPs could cause DNA fragmentation.
Key words: green synthesis; zinc oxide nanoparticles; silver nanoparticles; lemon peel; Cynara cardunculus. |
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Supervisor |
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Prof. Dr. Ekram Y. Danish |
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Thesis Type |
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Doctorate Thesis |
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Publishing Year |
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1445 AH
2023 AD |
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Added Date |
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Sunday, October 29, 2023 |
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Researchers
| مها غازي بترجي | Batterje, Maha Gazi | Researcher | Doctorate | |
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