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Document Type |
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Thesis |
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Document Title |
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EVALUATING GUT MICROBIOTA DIVERSITY IN SAUDI AUTISTIC CHILDREN تقييم التنوع الميكروبي في أمعاء الأطفال السعوديين المصابين بالتوحد |
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Subject |
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Faculty of Science |
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Document Language |
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Arabic |
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Abstract |
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Autism spectrum disorder (ASD) is a complicated neurodevelopmental illness described by decreased verbal and social interactions, limited interest and activities, and repetitive behaviors, with poorly understood underlying molecular mechanisms. However, several recent findings have linked changes in gut bacterial composition with the increased risk of ASD and highlighted the role of altering gut microbiota composition in the pathophysiology of autism disorder. Microbiota in the gut can effect brain function and performance throughout the microbiota-gut-brain axis. Therefore, the objective of the current analysis was to identify systemic alterations in the gut microbiome linked to autism. We recruited 11 non autistic children and 12 autistic children. We compared gut microbial community between ASD and neurotypical individuals in Saudi Arabia by sequencing the bacterial 16S rRNA gene. Results revealed that ASD patients had decreased in the a-diversity and marked alteration in gut microbiota composition compared to the neurotypical group. In this study, the butyrogenic gut microbiota strains were significantly lower in the gut microbiota of ASD patients compared to the control group. Notably, the presence of gut butyrogenic microbiota indicates good gastrointestinal (GI) health. We discovered that the ASD group had higher relative abundances of pathogenic bacteria such as, Escherichia-Shigella, Clostridium sensu stricto 1, and Clostridium_bolteae. On the other hand, Bifidobacterium spp.were less abundant in the ASD group than in the control group. PICRUSt analysis demonstrated some differential metabolites between the two groups. In compared to the control group, ASD individuals displayed enrichment in the purine and pyrimidine salvage genes and genes participating in the metabolism of neurotransmitter amino acids. In context, ASD patients showed a decline in the genes implicated in tryptophan metabolism and mitochondrial metabolism. Here, we suggested a new viewpoint of gut microbiota-mitochondria communication as a linking channel from the gut to the brain, which might assist in understanding the theory of gut-brain axis regulation. Therefore, modulating gut microbiota can pave the route for a new therapeutic approach to treating ASD.
Keywords: autism spectrum disorders ; ASD, intestinal microbiome ; microbiota-gut-brain axis; bacterial metabolism. |
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Supervisor |
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Prof. Dr. Ahmed Bahieldin Mohamed |
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Thesis Type |
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Doctorate Thesis |
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Publishing Year |
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1444 AH
2023 AD |
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Co-Supervisor |
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Dr. Najla Ali Alburae |
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Added Date |
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Wednesday, August 9, 2023 |
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Researchers
| أماني ساعد الحارثي | Alharthi, Amani Saed | Researcher | Doctorate | |
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