Document Details

Document Type : Thesis 
Document Title :
Evaluation of the immunogenicity of Adenovirus-based SARS-S1-CoV-2 vaccines in BALB/c mice
تقييم رد الفعل المناعي للقاح مبتكر قائم على الادينو فايروس ومستهدف للجزء البروتيني السطحي لفايروس S1 سارس2
 
Subject : Faculty of Science 
Document Language : Arabic 
Abstract : The development of safe and effective vaccine against the severe acute respiratory syndrome-Coronavirus 2 (SARS-CoV-2) become a global priority due to the ongoing pandemic of Coronavirus disease 2019 (COVID-19). Additionally, the emergence of SARS-CoV-2 variants has generated a need for more alternative vaccines using different strategies and/or antigens. In this study, we developed and assessed the immunogenicity of a novel recombinant replication-incompetent SARS-CoV-2 vaccines based on adenovirus serotype 5. Our generated construct expresses the CD40-targeted subunit 1 (S1) of SARS-CoV-2 spike protein (rAd5-SARS2-S1/tlhCD40L), and as a control we generated a construct expressing S1 alone (rAd5- SARS2-S1-S1), and as other one expressing Green Fluorescent Protein (rAd5-GFP). The efficacy and immunogenicity of our vaccine candidates have been evaluated in BALB/c mice. Intramuscular immunization with two doses of rAd5-SARS2-S1/tlhCD40L and rAd5- SARS2-S1-S1 induced robust and sustained specific immunoglobulin G (IgG), neutralizing antibodies (nAbs), B-cell and T-cell immune responses compared to rAd5-GFP. Although, both vaccine candidates could elicit strong humoral and cellular immune responses, integration of the molecular adjuvant CD40L enhanced the SARS-CoV-2-specific levels of IgG, nAbs, and B-cell. Importantly, we demonstrated that rAd5-SARS2-S1/tlhCD40L and rAd5- SARS2-S1-S1 induced cross-reactive neutralizing antibodies against SARS-CoV-2 variants of concerns. These results show that a replication-deficient human adenovirus serotype 5-based vaccine that expresses the S1 subunit fused to human CD40 ligand (CD40L) (rAd5-SARS2-S1/tlhCD40L) could be an effective potential vaccine for COVID-19. 
Supervisor : Dr. Wesam Hussain Abdulaal 
Thesis Type : Doctorate Thesis 
Publishing Year : 1444 AH
2022 AD 
Co-Supervisor : Prof. Anwar Mohammad Hashem 
Added Date : Monday, February 27, 2023 

Researchers

Researcher Name (Arabic)Researcher Name (English)Researcher TypeDr GradeEmail
نجوى ضيف الله الجهنيAljehani, Najwa DifAllahResearcherDoctorate 

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