Document Details
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Document Type |
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Thesis |
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Document Title |
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Effect of ethanolic extract of Alhagi maurorum roots on renal failure induced by acetaminophen in mice تأثير مستخلص الإيثانول لجذور نبات العاقول على الفشل الكلوي المستحدث بواسطة الأسيتامينوفين في الفئران |
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Subject |
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Faculty of Science |
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Document Language |
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Arabic |
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Abstract |
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Background: The goal of this research was to study protective actions of ethanol Alhagi maurorum (AM) root ethanol extract on acetaminophen-induced oxidative stress and renal toxicity in mice.
Methods: Forty male SWR strain albino mice aged 8 weeks were sorted into five groups. G1 (n= 5): is the control group. G2 (n= 5): administered orally a single dose of acetaminophen (2000 mg/kg). G3 (n=10) administrated orally 200 mg/kg of Alhagi maurorum roots (AM) roots ethanol extract for one week then acetaminophen as G2 at 8th day. G4 (n=10) administrated orally 400 mg/kg of Alhagi maurorum roots (AM) roots ethanol extract for one week then acetaminophen as G2 at 8th day. G5 (n=10) administrated orally 600 mg/kg of roots ethanol extract of Alhagi maurorum roots (AM) for one week then acetaminophen as G2 at 8th day. The initial and final body weights were measured. Mortality rate in different groups were calculated. At end of experiments (9th day), the mice were killed under anesthesia and blood samples were gathered to make complete blood picture (CBC), serum levels of urea and creatinine and oxidative stress biomarkers as glutathione (GSH), catalase (CAT) and superoxide dismutase (SOD) using available Elisa mice kits. Kidneys removed and subjected to histopathological examination.
Results: Initial body weights were significantly Higher in G2, G3 and G5 compared with G1; in G5 versus G2, G3 and G4 but were significantly Lower in G3 and G4 compared with G2; in G3 compared with G4. Final body weight was significantly increased in G2 and G5 compared with G1 (P <0.0001 and P <0.025) and in G5 compared with G3 and G4 (P <0.0001 for both) but significantly decreased in G4 versus G1 (P <0.0001); in G3, G4, G5 compared with G2 (P <0.0001, P <0.0001 and P <0.025); in G4 compared to G3 (P <0.0001). Weight gain and percentage changes in weight gain were significantly decreased in G2, G3, G4 and G5 versus G1 (P <0.0001); in G3, G4 and G5 versus G2 (P <0.0001) but were significantly elevated in G3 and G4 versus G5 (P <0.0001). The mortality rate was 40% in G2, G3 and G4 and 20% in G5. Meanwhile there was no mortality in G1. Acetaminophen intake significantly elevated WBCs, neutrophils, monocytes, urea and creatinine levels and significantly decreased RBCs, hemoglobin, hematocrit, GSH, SOD and CAT (P <0.05). Treatment with Alhagi maurorum roots extract specially high dose (600 mg/kg) resulted in decreased in WBCs, neutrophils, monocytes, urea and creatinine levels and significantly increased RBCs, hemoglobin, hematocrit, GSH, SOD and CAT versus acetaminophen group. Alhagi maurorum root extract treatment similarly decreased renal histological alteration induced by acetaminophen.
Conclusions: This study can be utilized as a prove of showing that Alhagi maurorum ethanol root extract treatment especially with high dose might be administered to prevent renal destruction induced by acetaminophen due to its antioxidant activity. |
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Supervisor |
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Prof. Mohammed Othman Aljahdali |
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Thesis Type |
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Master Thesis |
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Publishing Year |
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1443 AH
2022 AD |
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Added Date |
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Wednesday, January 4, 2023 |
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Researchers
| سهام ابراهيم النافع | Alnafe, Seham Ibrahim | Researcher | Master | |
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