Document Details
|
Document Type |
: |
Thesis |
|
Document Title |
: |
BIOMONITORING OF SELECTED ENVIRONMENTAL POLLUTANTS IN RELATION TO OSTEOPOROSIS: BIOCHEMICAL AND MOLECULAR CHARACTERIZATION الرصد الحيوي لملوثات بيئية مختارة فيما يتعلق بمرض هشاشة العظام: توصيف كيميائي حيوي و جزيئي |
|
Subject |
: |
faculty of science |
|
Document Language |
: |
Arabic |
|
Abstract |
: |
Exposure to environmental pollutants, including Perfluoroalkyl substances (PFASs) and heavy metals, causes deep concern regarding their potential adverse outcomes on the environment and human health owing to their unique biological characteristics. Several biomonitoring studies in many parts of the world investigated the impact of these contaminants on bone health. Osteoporosis is the most common type of bone disorders which affects an estimated 75 million people in the United States, Europe, and Japan. The case is not distinct in Saudi Arabia, in which an epidemiological study indicated that more than 30% of Saudi men and women, aged 50-79 years suffer from osteoporosis. Since Saudi Arabia is among the countries with the highest incidence of osteoporosis, identifying the risk factors of osteoporosis is essential to stop reduction of life quality and to diminish the high costs of treatment associated with this disorder. In addition to the environmental factors, bone density is under strong genetic control. Vitamin D receptor (VDR) is one of the most important genes studied in relation to osteoporosis. However, the correlation between VDR polymorphisms and bone mineral density (BMD) is controversial and has not been established in different ethnic populations. This study was aimed to analyze the association of selected PFASs and heavy metals concentrations with the incidence of osteoporosis. Furthermore, it evaluates the genetic association of VDR gene polymorphisms, particularly (Apa1 and Taq1), in osteoporotic Saudi patients. In this clinical case-control study, blood samples were collected from 200 participants (n = 100 osteoporosis and n = 100 healthy controls) aged ≥ 40 years from Jeddah, Saudi Arabia. Samples were analyzed for 4 types of PFASs by using Ultra Performance Liquid Chromatography -Tandem Mass Spectrometry (UPLC-MS/MS). Moreover, blood levels of heavy metals, including Pb, As and Cd, were assessed by Inductive Coupled Plasma Mass Spectrometry (ICP/MS). In addition, DNA was extracted for determining the genotypes and allele frequencies of VDR variants by polymerase chain reaction–restriction fragment length polymorphisms (PCR-RFLPs). Non-parametric Mann-Whitney Test, Pearson correlation and logistic regression were used to analyze PFASs and heavy metals results. Likewise, VDR polymorphisms were assessed using logistic regression, Non-parametric Mann-Whitney Test, and Hardy–Weinberg equilibrium. They showed that PFASs were not related to osteoporosis, whereas, all studied heavy metals were significantly higher in osteoporotic patients than that in controls, P ≤ 0.001.
Furthermore, a significant increase in the genotype frequencies of the ApaI (Aa) and (aa) was observed among osteoporotic patients compared to controls (P = 0.002 and P < 0.0001, respectively). Only the homozygous tt genotype of TaqI was significantly higher in those with osteoporosis than in controls (P = 0.001).
In conclusion, the results provide a support for the hypothesis that osteoporosis is a multifactorial disorder. In addition to genes, heavy metals constitute a real danger to bone health in this representative sample of adult Saudi population. |
|
Supervisor |
: |
Prof. Taha A. Kumosani |
|
Thesis Type |
: |
Doctorate Thesis |
|
Publishing Year |
: |
1441 AH
2020 AD |
|
Added Date |
: |
Wednesday, July 8, 2020 |
|
Researchers
| عبير عبدالرحمن بنجابي | Binjabi, Abeer Abdalrahman | Researcher | Doctorate | |
|
Back To Researches Page
|