Document Details

Document Type : Thesis 
Document Title :
FUNCTIONAL GENOMICS STUDIES IN MEDICINAL PLANT CATHARANTHUS ROSEUS”
"دراسات جينومية وظيفية على نبات الوينكا الطبي
 
Subject : Faculty of Science 
Document Language : Arabic 
Abstract : Medicinal plants are considered as a rich resource of pharmaceutical compounds that can be used for therapeutic purposes. Terpenoid Indole alkaloids (TIAs) such as vinblastine and vincristine in the medicinal plant Catharanthus roseus are two of the most widely used compounds in cancer chemotherapy against various leukemia, Hodgkin's disease and solid tumors. The main concerns about these compounds that their concentrations inside C. roseus organs tissues are quite low and their biosynthesis pathways are not fully characterized. Analyzing the transcriptome is the key to understand their biosynthesis’s mechanisms and improve their yields. Transcriptome data of C. roseus across different organs tissues was generated via Next-Generation Sequencing (NGS) technique. In this thesis, RNA-Seq data was collected from the National Center for Biotechnology Information (NCBI) database and de-novo assembled using Trinity and Tuxedo software packages. Then, the resulted differentially expressed (DE) transcripts were analyzed using different bioinformatics and experimental tools such as RSEM, EdgeR, and Blast-2-GO. Finally, results were validated using real time PCR technique. The outcomes indicated that DE transcripts related to vincristine and vinblastine accumulation are highly expressed in roots, flowers, and mature leaves tissues. Also, enzymes expressed from these DE transcripts are transferred from one organ tissues to another to open or complete the TIA pathway in the distal organs. The findings of this study will provide a new insight into genes and enzymes involved in regulating TIAs biosynthesis pathways. In addition, investigate the factors and mechanisms involved in assembling and facilitating the transport of these enzymes from one organ tissues to another. Moreover, the isolated genes from this study could facilitate in vitro overproduction of these compounds via metabolic engineering approaches. 
Supervisor : Dr. Ahmed M. Emara 
Thesis Type : Master Thesis 
Publishing Year : 1440 AH
2019 AD 
Co-Supervisor : Dr. Thana Khalid Khan 
Added Date : Tuesday, April 9, 2019 

Researchers

Researcher Name (Arabic)Researcher Name (English)Researcher TypeDr GradeEmail
منى عبدالقادرAbdulgader, Muna ResearcherMaster 

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