Document Details
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Document Type |
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Thesis |
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Document Title |
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Biochemical studies for enhancement lipase production using new strain isolated from Saudi Arabia دراسات كيميائية حيوية لتحسين إنتاج انزيم الليباز باستخدام سلالة جديدة معزولة من المملكة العربية السعودية |
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Subject |
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Faculty of Sciences |
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Document Language |
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Arabic |
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Abstract |
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Lipases are widely extracted from animals, plants and microorganisms. Most of the enzymes used in industries are of microbial origin. They catalyze the hydrolysis of esters for long chain aliphatic acids from glycerol at lipid and water. Several microorganisms are produced lipases by, viz., eucarya, fungi, actinomycetes, yeast, bacteria, archea, etc. Microbial produced lipases include: Bacillus, Penicillium, Staphylococcus, Geotrichum, Pseudomonas and Aspergillus . Production of extracellular bacterial lipases are influenced by cultivation parameters such as pH, temperature, dissolved oxygen and medium composition. Lipase producing bacteria were isolated from around a local Arabian-American Oil Company, Rabigh , Saudi Arabia. The results showed that one of 11 tested isolates was the best lipase producer (A1), by measuring lipase activity according to measuring the amount of fatty acid. The results showed that isolate A1 showed 98% identity with Bacillus cereus, it was the highest lipase producer, thus it was selected for more detailed studies. The effect of growth conditions on lipase production was also studied, and the results showed that, the best condition for the highest amount of lipase production was in the presence of dextrin as carbon source and di ammonium hydrogen phosphate as nitrogen source. The best results were obtained at 35 ̊C for 60 h and pH 6. This study focused on lipase enzyme improvement, which was partially purified by using the ammonium sulfate precipitation (80% saturation) and immobilizing the purified lipase enzyme in calcium alginate beads. The results revealed that the thermal stability of immobilized lipase was higher than free enzyme. The effect of metal ions Mg+2, Hg+2, Cu+2, Na+ and Ca+2 at a concentration 2 mmol used in the study showed inhibitory effect on immobilized more than the free lipase enzyme. These results highlighted the biochemical and technical advantages of immobilized lipase over the free enzyme. |
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Supervisor |
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Prof. Enas Nabil Danial |
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Thesis Type |
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Master Thesis |
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Publishing Year |
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1440 AH
2019 AD |
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Co-Supervisor |
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Prof. Najla Othman Ayaz |
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Added Date |
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Thursday, January 24, 2019 |
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Researchers
| تهاني طالع الرحيلي | Alrehaili, Tahani Talea | Researcher | Master | |
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