Document Details
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Document Type |
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Thesis |
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Document Title |
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BIOACTIVE METABOLITES OF SOME SAUDI RED SEA ALGAE نواتج حيوية فعالة بيولوجيا من بعض طحالب البحر الاحمر السعودى |
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Subject |
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Faculty of Marine Sciences |
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Document Language |
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Arabic |
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Abstract |
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Marine environment provides a rich source of chemical and biological diversity for bioactive constituents with desirable biological properties. In this study, we are interested to investigate the chemical composition and bioactivity of some Red Sea algae. Lipids, fatty acids, carbohydrates, proteins, and phenolic compositions from different Saudi Red Sea algae (red, green and brown) were determined in correlation with their metabolites as well as antibioactivity inhibitory agents. Chemical compositions were determined using Gas Chromatography-Mass Spectromatry GC-MS for lipoidal matters, High Performance Liquid Chromatography (HPLC) for phenolic, Gas Liquid Chromatography (GLC) for carbohydrate and UV Spectrophotometry for proteins. The lipoidal and phenolic contents were evaluated for their antimicrobial and antioxidant activities, respectively.
Results of lipids and fatty acids (FAs) reveled that palmitic acid was the predominant saturated FAs in all examined algae species, except C. trinodis which is rich in unsaturated oleic acid with 34.95%. Phytol was abundant in high level in H. macroloba at 53.99%, while cholesterol and stigmasterol as steroidal compounds were recorded in the best values at 29.1 and 23.4% from D. cervicornis and H. opuntia, respectively. D. cervicornis (brown algae) recorded to have the highest concentration of the total sugar at 15.75µg/g. On the other hand, T. expedition (green algae) recorded to have high content of conjugated fucose with 65.53%, followed by high significant amount of mannose at 55.67% in red alga G. debilis. The highest concentration of protein was found in T. oranata and D. simplex at 61.83 and 48.11 mg/g, respectively. The powerful antioxidant, gallic acid was detected in highest content in D. cervicornis at 227.5µg/g, compared to that in vegetables while kaempferol as flavonoid was recorded in p. gymnospora at 85.8µg/g. Moreover, the FAs contents of D. simplex exhibited antibacterial effect on S. typhimurium with MIC value 78.13 mg/mL. At the same MIC value, the FAME of J. rubens inhibited strongly the pathogenic fungus C. neoformas by 24.9 mm inhibition diameter. Of the green algae fractions, USM content of H. opuntia was the most potent antibacterial against the resistant bacteria K. Pneumonia with 18 mm inhibition diameter. MeOH extracts of the investigated algae showed mild to weak antioxidant activities indicated low concentrations of their phenolic compounds. |
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Supervisor |
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Prof. Dr. Usama Wahid Hawas |
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Thesis Type |
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Master Thesis |
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Publishing Year |
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1440 AH
2019 AD |
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Added Date |
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Tuesday, January 1, 2019 |
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Researchers
| ساره محمد حسين | Husain, Sarah Mohammed | Researcher | Master | |
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