Document Details
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Document Type |
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Thesis |
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Document Title |
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IDENTIFICATION OF NATURAL COMPOUNDS THAT INHIBIT PROTEIN GLYCATION IN DIABETIC RATS التـعرف على المركبات الطبيعية التي تثبط جلكزة البروتين في الجرذان المصابة بالسكري |
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Subject |
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Faculty of Sciences |
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Document Language |
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Arabic |
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Abstract |
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Glycation of proteins increases as blood glucose elevated in the body that slowly attacks a reactive amino group of proteins and impairs their functions, generates reactive oxygen species as a byproduct and finally generates advanced glycation end products (AGEs) which increases risk of cancer by interaction with receptor for advanced glycation end products (RAGE). This study evaluated the antiglycation, antioxidant, antiproliferative effects of bioactive peptides obtained from enzymatic hydrolysis of Vicia faba, soya bean, lens culinaris (red lentil, brown lentil), camel milk, cow milk, chicken, red sea hamour, red sea shrimp and red sea squid. Tryptic digestion of proteins was tested by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). Results obtained showed that; Vicia faba hydrolysate fractions <3 kDa were active. They trapped extra glucose and fructose, inhibited glycation of bovine serum albumin up to 49.5%, exhibited a cytotoxic effect against PC3, MCF-7 and HepG2 cell lines with a significant decrease in cell viability after 48 h of incubation and the minimum concentration to get 50% inhibitory activity ranged between 0.54 mg/ml to 7.58 mg/ml. Hydrolysate fractions <3 kDa from soya bean, lens culinaris (red lentil, brown lentil), camel milk, cow milk, chicken, red sea hamour, red sea shrimp and red sea squid also exhibited antiglycation and antioxidant potential by different concentration. More than 20 oligopeptide sequences were identified. According to this study, Vicia faba, soya bean, lens culinaris (red lentil, brown lentil), camel milk, cow milk, red sea hamour, red sea shrimp and red sea squid would be recommended as food ingredients or nutritional supplements due to its bioactive peptides contents.
Keywords: Glycation, Antioxidant, Antiproliferative, Bioactive peptides, MTT assay, HPLC-MS-MS. |
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Supervisor |
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Prof. Khalid Omar Abulnaja |
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Thesis Type |
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Doctorate Thesis |
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Publishing Year |
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1440 AH
2018 AD |
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Added Date |
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Tuesday, October 9, 2018 |
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Researchers
| عبدالقادر قربان | Kuerban, Abudukadeer | Researcher | Doctorate | |
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