Document Details
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Document Type |
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Thesis |
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Document Title |
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EFFECT OF AJWA DATES (PHOENIX DACTYLIFERA.L) EXTRACT ON PROLIFERATIION AND APOPTOSIS IN PROSTATE CANCER CELL LINE تأثير مستخلص تمر العجوة على النمو و الموت المبرمج لخلايا سرطان البروستاتا |
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Subject |
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Faculty of Sciences |
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Document Language |
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Arabic |
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Abstract |
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Phoenix dactylifera L. (Ajwa date) also known as date palm well known for its nutritive value around the world and consumed in Arabian peninsula as an essential diet. Ajwa date has been mention in prophetic, traditional and alternative medicine, for numerous health benefits, including antioxidant, antidiabetic, anticancer, antimicrobial, antihyperlipidemic, hepatoprotective, nephroprotective, neuroprotective, gastrointestinal and sexual improvement properties and many studies reported to have these properties. The aim of the study is to evaluate the anticancer effects of the extract of Ajwa date on human prostate cancer cell line (PC3).
PC3 cell line was treated with various concentrations (0.2, 0.3, 0.4, 0.5, and 0.6) of ethyl acetate fraction of Ajwa dates (EAFAD) to study their morphological and cellular changes and induction of apoptosis. Antiproliferative effect was measured using MTT assay. The long-term effect of EAFAD was determined using colony assay. Morphological changes like blebbing, vascularization, chromatin condensation and nuclear condensation were measured by different stains like Giemsa and fluorescent stains (DAPI and acridine orange/ Ethidium bromide). Loss of mitochondrial membrane potential and increased oxidative stress were measured using JC-1 and DCFH-DA dyes. DNA degradation was assayed by comet assay. Cell cycle distribution was measured by flow cytometer. The apoptotic cell was quantified by annexin V-FITC and propidium iodide dual staining using flow cytometer.
Culturing and characterization of tumor sphere were done for anti-metastatic studies of EAFAD. Tumor-sphere formation, chemo-resistance, migration and invasion assays are performed for confirmation of EAFAD as anti-metastatic. Anti-tumorigenic activity was measured using in-vivo mouse model. Collectively, the extract of EAFAD demonstrated strong anti-proliferative, apoptotic, anti-tumorogenic effect in a PC3 cell line in vitro and in vivo. Thus, the present study suggested EAFAD has potential therapeutics properties for prostate cancer. |
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Supervisor |
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Prof. Atef M. Al-Attar |
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Thesis Type |
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Doctorate Thesis |
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Publishing Year |
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1439 AH
2018 AD |
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Added Date |
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Wednesday, May 30, 2018 |
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Researchers
| مقتدر بايج ميرزا | Mirza, Muqtadir Baig | Researcher | Doctorate | |
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