Document Details
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Document Type |
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Thesis |
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Document Title |
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BIOCHEMICAL AND TOXICOLOGICAL EVALUATION OF ATORVASTATIN AND RIBOFLAVIN IN DIETHYLNITROSAMINE INDUCED HEPATOCELLULAR CARCINOMA التقييم الكيميائي الحيوي والسمي لأتورفاستاتين ورايبوفلافين في سرطان الدم لخلايا الكبد المستحث بداي إيثايل نيتروزأمين |
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Subject |
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Faculty of Sciences |
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Document Language |
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Arabic |
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Abstract |
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Background: Atorvastatin, a commonly prescribed drug for the management of hyperlipidemia, act as competitive inhibitors of HMG-CoA reductase—a rate-limiting enzyme in cholesterol synthesis. On the other hand riboflavin is also a well-studied micronutrient known for its anti-proliferative, anti-metastasis and antioxidant properties. However, the synergistic or antagonistic effect of both drugs when administered together is not studied yet.
Methodology: This study was an attempt to evaluate the toxicity/efficacy of atorvastatin (30mg/kg) in combination of riboflavin (50mg/kg) for 12 weeks in hepatocarcinogenic rats that were challenged by a single diethylnitrosamine (DENA) (160 mg/kg). Serum ALT, AST, CK, urea, uric acid, creatinine, bilirubin, albumin, LDL, FT3, FT4, calcium, phosphorus, triglyceride, glucose and vitamin D levels were estimated. Histopathology was also performed to study the alterations in the cellular architecture of cardiac and hepatic cells.
Results: The results revealed that DENA significantly altered (P < 0.001) most of the parameters when compared with normal control. Atorvastatin+Riboflavin group significantly managed to restore the altered parameters like LDH, cholesterol, triglycerides and LDL-C. Nonetheless this drug combination also caused mild hepatic damage by increasing the ALT, AST, total bilirubin and creatine kinase. But the observation that took us aback is tremendous increase in level of serum glucose and steep decline (P< 0.001) in vitamin D level by atorvastatin control when compared to normal control.
Conclusion: Though we initially hypothesized that statin and micronutrient combination may be able to treat the hepatocellular carcinoma, we conclude it as harmful therapy promoting the hepatic damage. Besides, long-term riboflavin intake or atorvastatin treatment may significantly lead to Vitamin-D deficiency and causes the persistent increase in the level of glucose. |
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Supervisor |
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Prof. Fahad A. Al-Abbasi |
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Thesis Type |
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Doctorate Thesis |
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Publishing Year |
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1439 AH
2018 AD |
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Co-Supervisor |
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Prof. Firoz Anwar |
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Added Date |
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Sunday, May 20, 2018 |
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Researchers
| كليم الدين محمد | Mohammed, Kaleemuddin | Researcher | Doctorate | |
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