Document Details

Document Type : Article In Journal 
Document Title :
Enhanced superoxide release and elevated protein kinase C activity in neutrophils from diabetic patients:in neutrophils from diabetic patients: association with periodontitis
Enhanced superoxide release and elevated protein kinase C activity in neutrophils from diabetic patients:in neutrophils from diabetic patients: association with periodontitis
 
Document Language : English 
Abstract : Inflammation and oxidative stress are important factors in the pathogenesis of diabetes and contribute to the pathogenesis of diabetic complications. Periodontitis is an inflammatory disease that is characterized by increased oxidative stress, and the risk for periodontitis is increased significantly in diabetic subjects. In this study, we examined the superoxide (02 -)-generating reduced nicotinamide adenine dinucleotide phosphate - oxidase complex and protein kinase C (PKC) activity in neutrophils. Fifty diabetic patients were grouped according to glycemic control and the severity of periodontitis. Neutrophils from diabetic patients with moderate [amount of glycated hemoglobin (HbA1c) between 7.0% and 8.0%] or poor (HbA1c >8.0%) glycemic control released significantly more O2- than neutrophils from diabetic patients with good glycemic con trol (HbA1c <7.0%) and neutrophils from nondiabetic, healthy individuals upon stimulation with 4pphorbol 12-myristate 13-acetate or N-formyl-MetLeu-Phe. Depending on glycemic status, neutrophils from these patients also exhibited increased activity of the soluble - and membrane-bound forms of PKC , elevated amounts of diglyceride, and enhanced phosphorylation of p4 7 -phox during cell stimulation. In addition, we report a significant correlation between glycemic control (HbA1c levels) and the severity of periodontitis in diabetic patients, suggesting that en hanced oxidative stress and increased inflanunation exacerbate both diseases. Thus, hyperglycemia can lead to a novel form of neutrophil priming, where elevated PKC activity results in increased phosphorylation ofp47-phox and O2- release.]. Leukoc. Biol. 78: 862-870; 2005. 
ISSN : 1 
Journal Name : Journal of Leukocyte Biology 
Volume : 87 
Issue Number : 1 
Publishing Year : 2005 AH
2005 AD 
Article Type : Article 
Added Date : Tuesday, May 20, 2008 

Researchers

Researcher Name (Arabic)Researcher Name (English)Researcher TypeDr GradeEmail
ممدوح كريمهKarima, Mamdouh InvestigatorDoctoratemkarimah@kau.edu.sa

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