Document Details

Document Type : Article In Journal 
Document Title :
Glomerular changes in microscopic haematuria, studied by quantitative immunoelectron microscopy and in situ zymography
Glomerular changes in microscopic haematuria, studied by quantitative immunoelectron microscopy and in situ zymography
 
Document Language : English 
Abstract : The ability of the fetal pancreatic islet cells to multiply rendered them a potential tissue for transplantation studies to cure diabetes. A bank of fetal islets could be created with proper storage in liquid nitrogen. The aim of this study is to evaluate the effect of thawing rate and post-thaw culture on the structural and functional integrity of isolated cryopreserved islets of rat fetuses. Fetal rat islets were isolated by the collagenase digestion, cultured for three days, and then cryopreserved using dimethylsulphoxide as cryoprotectant and the step-rate cooling to -40 degrees C before immersing them in liquid nitrogen. The islets were thawed by the slow or fast warming rates using hyperosmolar sucrose solution and then cultured for 1 or 2 days. Insulin and C-peptide contents of the slow thawed islets were higher than those of the control. In the fast thawed islets the contents were similar to those of the control. Insulin and C-peptide release in response to glucose for the slow thawed islets were lower than those of the control and in the fast thawed islets they were similar to that of the control. Histological examination showed irregular periphery and fragmented central part of the large slowly thawed islets, which showed also variable immunohistochemical reaction to anti-insulin serum, ranging from strongly positive reaction to markedly weak reaction. Fast thawed islets showed mostly regular periphery and their reaction to the anti-insulin serum was slightly weaker than that of the control islets. It was concluded that fast thawing and post-thaw culture is much better than slow thawing, as indicated by nearly normal insulin and C-peptide content and release and intact structural integrity. 
ISSN : 1460-2385 
Journal Name : Nephrology, dialysis, transplantation 
Volume : 17 
Issue Number : 9 
Publishing Year : 1423 AH
2002 AD 
Article Type : Article 
Added Date : Monday, July 5, 2010 

Researchers

Researcher Name (Arabic)Researcher Name (English)Researcher TypeDr GradeEmail
سوسن جللهJalalah, sawsan ResearcherDoctorate 
ابراهيم الزهرانيAlzahrani, Ibrahim ResearcherDoctorate 
بيتر فورنيسFurness, Petter ResearcherDoctorate 

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