Document Details Document Type : Article In Journal  Document Title : Glucose and glutamine metabolism in the small intestine of septic rats Glucose and glutamine metabolism in the small intestine of septic rats   Subject : Biochemistry  Document Language : English  Abstract : The intestinal metabolism of glucose and glutamine was studied in rats made septic by cecal ligation and puncture technique. Sepsis resulted in negative nitrogen balance and produced increases in the concentrations of blood pyruvate, lactate, alanine, and glutamine, and decreases in those of 3-hydroxybutyrate and acetoacetate. Both plasma insulin and glucagon concentrations were increased by 2.2- and 3.2-fold in septic rats, respectively. Portal-drained visceral blood flow increased in septic rats, and was accompanied by a decrease in the rates of utilization of glutamine and production of lactate, glutamate, and ammonia compared with those rates in sham-operated animals. Enterocytes isolated from septic rats showed decreased rates of glucose and glutamine utilization compared with cells isolated from corresponding controls. The maximal activities of hexokinase, 6-phosphofructokinase, pyruvate kinase, and glutaminase were decreased in intestinal mucosal scrapings of septic rats. It is concluded that a moderate form of sepsis decreases the rates of glucose and glutamine utilization (both in vivo and in vitro) by the epithelial cells of the small intestine. This may be caused by changes in the maximal activities of key enzymes in the pathways of glucose and glutamine metabolism in these cells as a metabolic adaptation to spare glucose and glutamine for use by other tissues  ISSN : 0022-2143  Journal Name : The Journal of Laboratory and Clinical Medicine  Volume : 115  Issue Number : 6  Publishing Year : 1410 AH 1990 AD  Article Type : Article  Added Date : Thursday, November 24, 2011  Researchers Researcher Name (Arabic) Researcher Name (English) Researcher Type Dr Grade Email محمد العرضاوي Ardawi, Mohammed Investigator Doctorate   ياسر جمال Jamal, Yasir Researcher Doctorate   عبدالرحمن آشي Ashy, AbdulRahman Researcher Doctorate   Files File Name Type Description  31318.pdf pdf   Back To Researches Page