Document Details

Document Type : Article In Journal 
Document Title :
DNA barcode through cytochrome b gene information of mtDNA in native chicken strains
DNA barcode through cytochrome b gene information of mtDNA in native chicken strains
 
Document Language : English 
Abstract : This study was carried out to figure out the potentiality of a cytochrome b gene as a barcoding tool in discriminating native chicken strains and other Gallus gallus species. We performed PCR amplification using universal primer to amplify around 415 bp fragment of cytochrome b gene of mtDNA. The results revealed that all Saudi chicken strains were identical to each other but when compared with other species of Gallus the differences were exciting. The phylogenetic tree revealed that there were seven clusters represented for native strains and were clustered together especially in black strain and dark brown ones. The results have confirmed that using cytochrome b gene to discriminate between Saudi chicken strains and other species of G. gallus fowl was a very sufficient tool. Moreover, we can consider short fragment of cytochrome b gene of mtDNA as a universal DNA barcode region. It was a much more accurate and efficient tool to discriminate interspecies than intraspecies. We think it needs more studies to confirm this concept, and we have to apply that tool for many species of vertebrate and invertebrate as well. 
ISSN : 1940-1736 
Journal Name : MITOCHONDRIAL DNA 
Volume : 24 
Issue Number : 5 
Publishing Year : 1434 AH
2013 AD 
Article Type : Article 
Added Date : Tuesday, July 25, 2017 

Researchers

Researcher Name (Arabic)Researcher Name (English)Researcher TypeDr GradeEmail
Haitham YacoubYacoub, Haitham Researcher  
Moataz FathiFathi, Moataz Researcher  
Wael MahmoudMahmoud, Wael Researcher  

Files

File NameTypeDescription
 42230.pdf pdf 

Back To Researches Page