Document Details

Document Type : Article In Journal 
Document Title :
Development of Mediator-Free Acetylcholine Biosensor Based on Acetylcholine Oxidase Immobilized Micro-Chips
Development of Mediator-Free Acetylcholine Biosensor Based on Acetylcholine Oxidase Immobilized Micro-Chips
 
Document Language : English 
Abstract : Acetylcholine (ACh) biosensor is developed based on mediator-free acetylcholine oxidase (AChOx) by self-assembled monolayer (SAM) onto lab-made micro-chip. The simple cyclic voltammetry (CV) method is utilized in mediator-free system in phosphate buffer solution (PBS, 0.1M) at room conditions. The analytical parameters of AChOx fabricated electrode employed a lower detection limit (DL, 0.136 nM), a wide linear dynamic range (LDR, 1.0 nM to 1.0 mM), good linearity (R= 0.9991), and higher sensitivity (2.7329 mu AmM-1 cm(-2)) where a tiny sample volume (70.0 mu L) is required. The micro-chip system executes a simple and efficient approach to immobilize the enzymes onto SAM modified surface, which can improve the biosensor performances for a large group of biomolecules in broad scale of biomedical applications in health-care fields. 
ISSN : 1570-1646 
Journal Name : CURRENT PROTEOMICS 
Volume : 9 
Issue Number : 4 
Publishing Year : 1433 AH
2012 AD 
Article Type : Article 
Added Date : Sunday, July 9, 2017 

Researchers

Researcher Name (Arabic)Researcher Name (English)Researcher TypeDr GradeEmail
M RahmanRahman, M Researcher  

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