Document Details

Document Type : Article In Journal 
Document Title :
Characterization of a spontaneous nonmagnetic mutant of Magnetospirillum gryphiswaldense reveals a large deletion comprising a putative magnetosome island
دراسة خصائص طفرة غير مغنطيسية من Magnetospirillum gryphiswaldense تظهر إزالة كبيرة لجزيرة مغنطيسية
 
Document Language : English 
Abstract : Abstract Frequent spontaneous loss of the magnetic phenotype was observed in stationary-phase cultures of the magnetotactic bacterium agnetospirillum gryphiswaldense MSR-1. A nonmagnetic mutant, designated strain MSR-1B, was isolated and characterized. The mutant lacked any structures resembling magnetosome crystals as well as internal membrane vesicles. The growth of strain MSR-1B was impaired under all growth conditions tested, and the uptake and accumulation of iron were drastically reduced under iron-replete conditions. A large chromosomal deletion of approximately 80 kb was identified in strain MSR-1B, which comprised both the entire mamAB and mamDC clusters as well as further putative operons encoding a number of magnetosomeassociated proteins. A bacterial artificial chromosome clone partially covering the deleted region was isolated from the genomic library of wild-type M. gryphiswaldense. Sequence analysis of this fragment revealed that all previously identified mam genes were closely linked with genes encoding other magnetosome-associated proteins within less than 35 kb. In addition, this region was remarkably rich in insertion elements and harbored a considerable number of unknown gene families which appeared to be specific for magnetotactic bacteria. Overall, these findings suggest the existence of a putative large magnetosome island in M. gryphiswaldense and other magnetotactic bacteria. 
ISSN : 5779-5790 
Journal Name : JOURNAL OF BACTERIOLOGY 
Volume : 185 
Issue Number : 19 
Publishing Year : 1424 AH
2003 AD 
Article Type : Article 
Added Date : Saturday, September 3, 2011 

Researchers

Researcher Name (Arabic)Researcher Name (English)Researcher TypeDr GradeEmail
محمد حسين مدكورMadkour, Mohamed HusseinResearcherDoctoratemadkour55@yahoo.com

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