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Document Type |
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Article In Journal |
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Document Title |
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MECHANISMS OF MULTIPLE-DRUG RESISTANCE IN PSEUDOMONAS AERUGINOSA MECHANISMS OF MULTIPLE-DRUG RESISTANCE IN PSEUDOMONAS AERUGINOSA |
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Document Language |
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English |
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Abstract |
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Objective: Mechanisms of multiple drug resistance in Pseudomonas aeruginosa.
Materials and Methods: A total of six spontaneous ciprofloxacin-resistant mutants were isolated from Pseudoinonas aeruginosa clinical isolate on agar plates containing ciprofloxacin. Five of these mutants (PaMl, PaM2, PaM4-6) are characterized by cross-resistance to other fluoroquinolones (norfioxacin and ofloxacin), B-lactam antibiotics (cefamandole, cefoperazone, ceftazidime, ceftriaxone, and cefepime), and aztreonam. While the PaM3 mutant is characterized by selective resistance to the tested fluoroquinolones.
Results: The five multiple-drug resistant mutants had about 3.4- to 5.5-time lower rate of norfioxacin uptake than that of the parent isolate, while no significant change in norfloxacin uptake was observed in case of PaM3 mutant. Addition of 0.25 mM carbonyl cyanide m-chlorophenylhydrazone increased the norfloxacin uptake by only PaM2 mutant, while no change was observed in case of the other mutants. The outer membrane proteins (OMPs) analysis showed a significant reduction of 41 and 42 KDa OMP in PaM1, PaM4, PaM5, and PaM6 and overproduction of 50 KDa and disappearance of 41 KDa OMPs in PM2 mutant as compared to the parent isolate. In case of mutant PaM3 no significant change in the OMP profile was observed. The results of 13-lactamase induction and DNA gyrase assay showed no difference between the selected mutants and their parent, with exception for PaM3 mutant in which a significant decrease in the DNA gyrase sensitivity to ciprofloxacin was observed.
Conclusion: Reduction or disappearance of 41 and 42 KDa and overexpression of 50 KDa OMPs in P. aeruginosa isolates is associated with cross-resistance to fluoroquinolones, β -lactam antibiotics , and aztreonam. |
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ISSN |
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1110-1431 |
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Journal Name |
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Arab. J. Lab. Med |
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Volume |
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35 |
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Issue Number |
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1 |
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Publishing Year |
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1429 AH
2009 AD |
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Article Type |
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Article |
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Added Date |
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Sunday, May 30, 2010 |
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Researchers
| سليمان الشيخ | EL-sheikh, Soliman | Researcher | Doctorate | |
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