Document Details

Document Type : Article In Journal 
Document Title :
Novel Mutations in the Displacement Loop of Mitochondrial DNA are Associated with Acute Lymphoblastic Leukemia: A Genetic Sequencing Study
Novel Mutations in the Displacement Loop of Mitochondrial DNA are Associated with Acute Lymphoblastic Leukemia: A Genetic Sequencing Study
 
Document Language : Arabic 
Abstract : Background: Acute lymphoblastic leukemia (ALL) is the most common cancer diagnosed in children and represents approximately 25% of cancer diagnoses among those younger than 15 years of age. Materials and Methods: This study investigated alterations in the displacement loop (d-loop) region of mitochondrial DNA (mtDNA) as a risk factor and diagnostic biomarker for early detection and diagnosis of acute lymphoblastic leukemia. Using mtDNA from 23 subjects diagnosed with acute lymphoblastic leukemia, the first 450 bp of the d-loop region were amplified and successfully sequenced. Results: This revealed 132 mutations at 25 positions in this region, with a mean of 6 alterations per subject. The d-loop alterations in mtDNA in subjects were all identified as single nucleotide polymorphisms in a homoplasmic distribution pattern. Mutant alleles were observed in all subjects with individual frequency rates of up to 95%. Thirteen mutant alleles in the d-loop region of mtDNA occurred with a high frequency. Novel alleles and locations were also identified in the d-loop of mtDNA as follows: 89 G insertions (40%), 95 G insertions (13%), 182 C/T substitutions (5%), 308 C insertions (19%), and 311 C insertions (80%). The findings of this study need to be replicated to be confirmed. Conclusions: Further investigation of the relationship between mutations in mitochondrial d-loop genes and incidence of acute lymphoblastic leukemia is recommended. 
ISSN : 1513-7368 
Journal Name : ASIAN PACIFIC JOURNAL OF CANCER PREVENTION 
Volume : 15 
Issue Number : 21 
Publishing Year : 1435 AH
2014 AD 
Article Type : Article 
Added Date : Tuesday, August 1, 2017 

Researchers

Researcher Name (Arabic)Researcher Name (English)Researcher TypeDr GradeEmail
Haitham YacoubYacoub, Haitham Researcher  
Wael MahmoudMahmoud, Wael Researcher  
Hatim El-BazEl-Baz, Hatim Researcher  
Ola EidEid, Ola Researcher  
Refaat ELfayoumiELfayoumi, Refaat Researcher  
Salem ElhamidyElhamidy, Salem Researcher  
Maged MahmoudMahmoud, Maged Researcher  

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