Document Details

Document Type : Article In Journal 
Document Title :
Production of salinity tolerant Nile tilapia, Oreochromis niloticus through traditional and modern breeding methods: II. Application of genetically modified breeding by introducing foreign DNA into fish gonads
Production of salinity tolerant Nile tilapia, Oreochromis niloticus through traditional and modern breeding methods: II. Application of genetically modified breeding by introducing foreign DNA into fish gonads
 
Subject : Biological 
Document Language : English 
Abstract : This study was conducted to produce a salinity tolerant Nile tilapia, Oreochromis niloticus through genetically modified breeding by introducing a fragmented purified DNA isolated from sea bream, Sparus aurata or Artemia, Artemia salina into the gonads. The results showed a significant improvement (P ≤ 0.05) in most of the growth performance and feed utilization parameters of genetically modified O. niloticus treated with sea bream-DNA and reared at different salinity levels up to 16 ppt compared to both genetically modified O. niloticus treated with Artemia-DNA and the control fish reared at the same salinity levels. Genetically modified O. niloticus treated with Artemia-DNA reared at 32 ppt, had displayed better traits results (P≤ 0.05) compared to the other fish within the same salinity level. Furthermore, genetically modified O. niloticus treated with sea bream-DNA showed a silver color covering all the body and no dark vertical bands. The results of the random amplified polymorphic DNA (RAPD) fingerprinting showed highly genetic polymorphic percentage (35.95%) among fish receiving foreign DNA and their control using different random primers. The results of the present work suggested that, hyper-saline genetically modified O. niloticus with higher growth rate can be produced using a feasible and fast methodology. 
ISSN : 1684-5315 
Journal Name : African Journal of Biotechnology 
Volume : 10 
Issue Number : 4 
Publishing Year : 1432 AH
2011 AD 
Article Type : Article 
Added Date : Saturday, June 16, 2012 

Researchers

Researcher Name (Arabic)Researcher Name (English)Researcher TypeDr GradeEmail
Samy Yehya El-ZaeemEl-Zaeem, Samy YehyaResearcherDoctorateselzaeem@ksu.edu.sa
محمد مرسي محمد أحمدAhmed, Mohamed Morsi M.ResearcherDoctorate 
Mohamed El-Sayed SalamaSalama, Mohamed El-SayedResearcherDoctorate 
Hamad Abdel-Razek El-MaremieEl-Maremie, Hamad Abdel-RazekResearcherDoctorate 

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