تفاصيل الوثيقة

نوع الوثيقة : مقال في مجلة دورية 
عنوان الوثيقة :
Production, purification and characterization of α-amylase from Trichoderma harzianum grown on mandarin peel
Production, purification and characterization of α-amylase from Trichoderma harzianum grown on mandarin peel
 
الموضوع : كيمياء حيوية 
لغة الوثيقة : الانجليزية 
المستخلص : The production, purification and characterization of α-amylase from Trichoderma harzianum grown on mandarin peel were investigated. The effect of incubation time and mandarin peel concentration on the production of α-amylase by T. harzianum was studied. α-Amylase A3 was purified from T. harzianum to electrophoretic homogeneity by using DEAE-Sepharose and Sephacryl S-200 columns. The enzyme had molecular weight of 70 kDa using gel filtration and SDS-PAGE. The affinity of the substrates toward A3 was in the order of amylopectin > glycogen > starch > β-cyclodextrin > dextrin > α-cyclodextrin. These findings tend to suggest that the enzyme has high affinity toward high-molecular mass substrates. The K m and V max values of the enzyme for hydrolyzing potato soluble starch and glycogen were 6.53, 4.5 mg/ml and 2 and 2.2 μmol reducing sugar/ml, respectively. The maximum activity of enzyme against soluble starch was determined at pH 4.5 and 40°C. α-Amylase A3 was stable up to 40°C for 30 min of incubation and retained 70 and 50% of its activity at 50 and 60°C, respectively. While all the examined metal cations were effective in inhibiting the enzyme, Ca 2+ considerably enhanced the activity. The metal chelators, EDTA, sodium citrate and sodium oxalate had inhibitory effects on A3. The rate of breakdown of starch was higher than the rate of formation of reduced sugar indicating A3 is endoacting enzyme. These properties of A3 with its remarkable activity meet the prerequisites needed for liquefaction and saccharification of starch industry. 
ردمد : 1996-0808 
اسم الدورية : African Journal of Microbiology Research 
المجلد : 5 
العدد : 8 
سنة النشر : 1432 هـ
2011 م 
نوع المقالة : مقالة علمية 
تاريخ الاضافة على الموقع : Monday, February 13, 2012 

الباحثون

اسم الباحث (عربي)اسم الباحث (انجليزي)نوع الباحثالمرتبة العلميةالبريد الالكتروني
صالح أحمد محمدMohamed, Saleh Ahmedباحثدكتوراهsaleh38@hotmail.com
عصام إبراهيم أزهرAzhar, Esam Iباحثدكتوراه 
مروج محمد باعكضهBa-Akdah, Morooj Mباحثماجستير 
Nisreen R TashkandyTashkandy, Nisreen Rباحثدكتوراه 
طه عبدالله قمصانيKumosani, Taha Abdullahباحثدكتوراهtkumosani@kau.edu.sa

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اسم الملفالنوعالوصف
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