Document Details
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Document Type |
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Thesis |
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Document Title |
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Effect of Usnic Acid (UA) and Conjugated Linoleic Acid (CLA) on Adiposity and BodWeight of Ratsies تأثير حمض الأوزنيك وحمض اللينولييك المتبادل على شحمية وأوزان الجرذان |
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Subject |
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Rats--Metabolism |
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Document Language |
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English |
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Abstract |
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Usnic acid (UA), which was extracted from a native lichen species Usnea articulata growing on juniper trees Juniperus procera in the Asir region, Saudi Arabia, and conjugated linoleic acid (CLA) were used to evaluate their effectiveness in body weight loss. A detailed cohort investigation accompanied this study to ensure the safety of these natural products on health and liver vitality.
The Usnea articulata extract contained 0.16 % ± 0.03 (+)-UA, which was found to reach a peak in rat plasma within 6 hr after oral administration of 500 mg UA/kg and remained in circulation for a period of 72 hr. Adult male Sprague-Dawley rats (SD) weighing 150–218 g were given oral UA suspended in 1% carboxymethyl cellulose (CMC)-water solution at two dosages, 100 mg and 300 mg/kg. Body weight gain, BMI, food intake, water intake, liver index and perirenal adipose tissue index were investigated. Serum composition including GLU, Mg, TP, ALB, ALP, AST, ALT, GGT, TBI, LDH, TG, CHOL, LDL, HDL, lipase, leptin, insulin and glutathione were determined. Histological study of perirenal adipocytes were carried out using H & E stained paraffin sections. Hepatocytes were also studied using transmission electron microscopy. CLA was given to another animal group at a dose of 5% and similar parameters were investigated. Data are expressed as mean ± SE and were analyzed by one-way analysis of variance (ANOVA) and t-tests as appropriate. An insignificant reduction in body weight gain, BMI, perirenal adipose tissue index, glutathione, leptin, and insulin were observed in UA-treated rats in comparison with the control. In contrast, liver index and food and water intake were increased. Serum ALP, AST, and LDH were decreased, whereas TP, ALT, TBIL, serum lipid profile, and lipase were significantly increased. No remarkable changes in body weight gain despite reduction in BMI gain were observed in animals receiving CLA. Food and water intake and perirenal adipose tissue were slightly decreased while the liver index was significantly increased. On the other hand, GLU, ALT and TBIL were decreased insignificantly. Leptin and other serum parameters were insignificantly increased, except for Mg, HDL, and lipase, which increased significantly. Morphological and morphometric reduction in adipocytes’ areas, with an increase in cell number, were observed in both UA and CLA groups. UA resulted in ultrastructure and morphometric changes in hepatocyte mitochondria (swellings and increased circumference with decrease in cristae). Nuclear membrane irregularities and increased heterochromatin were observed at the high dose.
In conclusion, UA has a dose-dependent effect on body weight, liver and perirenal adipose tissue indices, and liver enzymes with slight changes in hepatocyte mitochondria. Nanotechnology could be used in the future for safe preparation of UA formulations acting only on body fat stores. |
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Supervisor |
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. Ali Ahmed Al-Robai |
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Thesis Type |
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Doctorate Thesis |
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Publishing Year |
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1430 AH
2009 AD |
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Number Of Pages |
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166 |
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Co-Supervisor |
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Ahmed Nabil Abo-Khatwa |
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Added Date |
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Monday, May 19, 2014 |
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Researchers
| أحلام عبد العزيز الأحمدي | Al-Ahmadi, Ahlam Abdulaziz | Investigator | Doctorate | |
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Files
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36962.pdf
| pdf | شطر الطالبات |
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